Standard Operating Procedures (SOP)

Science is a discipline that depends on collaboration and the sharing of information. That is why the Streeck Lab is in the process of adapting their SOPs for the general public. The SOPs for several of the immunological and molecular techniques the Streeck Lab uses can be found below. If a specific technique is not found below, check back at a later date as the list will be updated over time.

 

GeneralFlow CytometryAssays
SOP - Z-score (standard score) calculation

04-00 MEDIA PREPARATION

04-01 IL-2 RECONSTITUTION

04-02 PHA RECONSTITUTION

05-00 DNA MEASURMENT WITH BIOSPECTROPHOTOMETER

11-00 SDS-PAGE GEL

11-01 WESTERN BLOTTING

12-00 MANUAL CELL COUNTING

24-00 dNTP ALIQUOTS

25-00 AGAROSE GEL ELECTROPHORESIS
06-00 ICS

06-01 TETRAMER STAINING

06-02 COMPENSATION BEADS

06-03 ANTIBODY TITRATIONS

10-00 CFSE STAINING

09-00 ELISPOT ASSAY

14-00 CHROMIUM ASSAY

15-00 NK DEGRANULATION ASSAY

16-00 VIRAL INHIBITION ASSAY

21-00 PERKIN ELMER P24 ELISA KIT PROTOCOL

22-00 LUMINEX
Cell CultureVirus Harvesting/CultureCell/Blood Processing
02-00 CTL FEEDING

02-01 CTL CLONING

02-02 RESTIMULATION

02-03 T CELL LINE GENERATION

02-04 CD4 HIV PEPTIDE SPECIFIC CLONES

07-00 MONOCYTE DERIVED DCs_MACROPHAGES

03-00 EBV TRANSFORMATION

17-00 HIV VIRUS CULTURING FROM PLASMA,SPIN PROTOCOL

18-00 HIV VIRUS CLONING AND TITERING

19-00 CULTURING HIV VIRUS FROM PBMC

20-00 PROPAGATION OF PRIMARY HIV-1 ISOLATES

08-00 THAWING PBMCs

08-01 FREEZING PBMCs

13-01 PBMC ISOLATION - LEUKAPHARESIS

13-00 PBMC ISOLATION - FICOLL HIV+

13-00 PBMC ISOLATION - FICOLL HIV-

26-00 THP-1 CELL LINES

27-00 FLOW PANEL DESIGN

28-00 TONSIL PROCESSING

31-00 Blood Processing
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